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Videos : Alkitabpenuntunhidupberkelimpahanpdfdownload.The level of mRNA encoded by the gonadotropin beta-subunit gene increases as preovulatory follicles mature in female rats.
In order to investigate the mechanism of preovulatory gonadotropin (luteinizing hormone [LH], follicle stimulating hormone [FSH]) release from the pituitary, the expression of mRNA for gonadotrophin-beta-subunits in the pituitary was investigated in vivo. Adult female rats were injected with PMSG 48 and h later with hCG and animals were killed at intervals during development of the follicles to study the expression of mRNA for gonadotrophin-beta-subunit by Northern hybridization. The apparent steady-state level of LH beta-subunit mRNA did not change in the course of a cycle. However, significantly higher levels were detected during pregnancy and an increase was observed during the 5 days before ovulation. All animals tested prior to the PMSG injection showed a greater expression of LH beta-subunit mRNA than that observed in pregnant animals tested 13 days after hCG. When the animals were killed 7 days after hCG a greater expression of LH beta-subunit mRNA was again found in pregnant animals. No change in the apparent steady-state level of FSH beta-subunit mRNA was observed during development of the follicles or during the changes observed in the LH beta-subunit mRNA level in the pituitary. In all of the animals tested before hCG (day 0) the level of FSH beta-subunit mRNA was lower than that of LH beta-subunit mRNA. It is concluded that the increase in the level of LH beta-subunit mRNA in the pituitary in the course of preovulatory follicle development is primarily a result

https://colab.research.google.com/drive/1PYZo36FOC50Z_Vguj9LC5aCUW0k2K2Qe
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https://colab.research.google.com/drive/1KHpqPIaO30hwmF1pw5jaNzvV9r2se-gv
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